{"id":19,"date":"2015-10-18T23:35:00","date_gmt":"2015-10-18T23:35:00","guid":{"rendered":""},"modified":"2015-10-28T17:10:57","modified_gmt":"2015-10-28T17:10:57","slug":"p1b-type-heavy-metal-atpase-transporters","status":"publish","type":"page","link":"https:\/\/grad.biology.ualberta.ca\/taylor\/p1b-type-heavy-metal-atpase-transporters\/","title":{"rendered":"P1B-type heavy-metal ATPase transporters"},"content":{"rendered":"<h2 style=\"line-height: 125%;\">Characterization of P<sub style=\"font-size: 0.8em;\">1B<\/sub>-type heavy-metal ATPase transporters in <em>Brachypodium distachyon<\/em><\/h2>\n<p>Funded by NSERC Discovery Grants Program.<\/p>\n<p><!--\n\nATP-binding cassette (ABC) proteins constitute a large, diverse and ubiquitous superfamily that uses\nthe hydrolysis of ATP to energize export or import of a wide range of molecules\nfrom cells. Members of the multidrug resistance-associated protein (MRP\/ABCC)\nsubfamily are membrane-bound proteins that transport glutathione-conjugate or\nmulti-specific organic anions across membranes. In plants, ABCCs are active in\nthe vacuolar transport of a range of compounds, including chlorophyll\ncatabolites, herbicides, and cadmium. There are 15 ABCCs in <em>Arabidopsis<\/em> and 17\nABCCs in rice. While there has been progress in functional characterization of <em>Arabidopsis<\/em> ABCCs, none have been\ncharacterized in rice. Little is known about the roles of ABCCs in\nagronomically important species, such as rice and wheat. The goal of this\nresearch is to understand how cereal ABCCs are involved in abiotic stress\ntolerance and cellular detoxification. The challenges of cloning ABCCs from\nwheat are two-fold: the complete wheat genome is unsequenced, and sequence\ncoverage and gene model assembly for wheat ABCCs is poor. Like rice, there are\nprobably at least 17 ABCC genes in wheat, plus homoeologous copies. To-date, we\nhave successfully cloned five full-length ABCC cDNAs from wheat and two\nhomologs from rice. We are using budding yeast, <em>Saccharomyces cerevisiae<\/em>, as a model system to systematically query\nhow individual ABCCs function at the cellular level. Yeast has homologous ABCC\ngenes for which knockout mutants are available. Complementation of the mutants\nthrough heterologous expression of the cereal ABCC genes allows us to rapidly\nquery the role of each cereal ABCC, including identification of the substrate\nspecificity and biochemical properties of the ABCC transporters.\n\n--><\/p>\n<div style=\"background-color: #f5f5f5; border: 1px solid #DDD; color: #333; padding: 0.5em 1em; border-radius: 5px; box-shadow: 3px 3px 5px rgba(204,204,204,0.3); margin: 1em auto; text-align: center;\"><strong>Characterization of P<sub style=\"font-size: 0.8em; line-height: 0;\">1B<\/sub>-type heavy-metal ATPase transporters in <em>Brachypodium distachyon<\/em><br \/>\nOpportunities for <a href=\".\/graduate-studies\">Graduate<\/a> and <a href=\".\/undergraduate-projects\">Undergraduate<\/a> research<\/strong><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" style=\"margin-bottom: 5px; display: block; margin-left: auto; margin-right: auto;\" title=\"Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon\" src=\"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-content\/uploads\/sites\/41\/2015\/10\/ABC-HMA-GJT-Web-Image.jpg\" alt=\"Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon\" width=\"626\" height=\"122\" \/><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon Funded by NSERC Discovery Grants Program. Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon Opportunities for Graduate and Undergraduate research &nbsp;<\/p>\n","protected":false},"author":60,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-19","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/users\/60"}],"replies":[{"embeddable":true,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/comments?post=19"}],"version-history":[{"count":5,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/19\/revisions"}],"predecessor-version":[{"id":174,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/19\/revisions\/174"}],"wp:attachment":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/media?parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}