{"id":150,"date":"2015-10-28T15:55:41","date_gmt":"2015-10-28T15:55:41","guid":{"rendered":"https:\/\/grad.biology.ualberta.ca\/taylor\/?page_id=150"},"modified":"2015-10-28T16:59:21","modified_gmt":"2015-10-28T16:59:21","slug":"graduate-studies","status":"publish","type":"page","link":"https:\/\/grad.biology.ualberta.ca\/taylor\/graduate-studies\/","title":{"rendered":"Graduate Studies"},"content":{"rendered":"<h2 style=\"line-height: 125%\">Graduate Research Opportunity<\/h2>\n<p>I am looking for a M.Sc. or Ph.D. student to undertake functional characterization of P<sub style=\"font-size: 0.8em line-height\">1B<\/sub>-type heavy-metal ATPase transporters from <em>Brachypodium distachyon<\/em> or wheat.<\/p>\n<p><strong>Details to be announced<\/strong><\/p>\n<p><!--\n\n\n<p>We have found that several cereal ABC genes from the multidrug resistance-associated protein (MRP\/ABCC) subfamily partially complement the Cd-hypersensitive yeast mutant, <em>ycf1&#916;<\/em>, suggesting these ABCCs exhibit some transport activity for glutathionylated compounds. These results raise many questions:<\/p>\n\n\n\n\n<ul>\n\n\n<li style=\"margin-bottom: 3px\">Are the ABCCs targeted to the tonoplast?<\/li>\n\n\n\n\n<li style=\"margin-bottom: 3px\">What are the substrates transported by the ABCC proteins?<\/li>\n\n\n\n\n<li style=\"margin-bottom: 3px\">Are the cereal ABCCs able to rescue <em>ycf1&#916;<\/em>'s sensitivity to lead and arsenate?<\/li>\n\n\n\n\n<li style=\"margin-bottom: 3px\">For ABCCs that do not complement <em>ycf1&#916;<\/em>, is the complete protein being expressed and correctly targeted <em>in vivo<\/em>?<\/li>\n\n\n\n\n<li style=\"margin-bottom: 3px\">Where are ABCCs expressed <em>in planta<\/em>?<\/li>\n\n\n\n\n<li style=\"margin-bottom: 3px\">Is expression of cereal ABCCs influenced by abiotic stress?<\/li>\n\n\n\n\n<li>Are homoeologous copies of ABCCs functionally redundant?<\/li>\n\n<\/ul>\n\n\n--><\/p>\n<p>Master&#8217;s or Ph.D. student projects will address some of these questions and would be tailored to the student&#8217;s interests and background.<br \/>\nA B.Sc. in plant biology, microbiology, cell biology, genetics, or a related discipline is essential.<br \/>\nPrevious coursework or laboratory experience in molecular biology and microbiology is an asset.<br \/>\nIf you are interested or have further questions, please contact Dr Taylor (<a href=\"mailto:gregory.taylor@ualberta.ca\">gregory.taylor@ualberta.ca<\/a>).<\/p>\n<p>Interested students will need to meet the Department of Biological Sciences <a href=\"\/programs\/graduate\/prospective\/?Page=4511\">entry standards<\/a> and qualify for funding support (e.g. Departmental Teaching Assistantship, NSERC studentship).<br \/>\nAdditional information for prospective students is available on the <a href=\"\/programs\/graduate\/prospective\/\">Biological Sciences website<\/a>.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" style=\"margin-bottom: 5px; display: block; margin-left: auto; margin-right: auto\" alt=\"Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon\" title=\"Characterization of P1B-type heavy-metal ATPase transporters in Brachypodium distachyon\" src=\"\/faculty\/gregory_taylor\/uploads\/images\/ABC-HMA-GJT-Web-Image.jpg\" height=\"122\" width=\"626\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><!--<span class=\"green14\"><strong>Graduate Student Opportunities<\/strong><\/span>\n\n\n<p>I am currently looking for students for the following projects:<\/p>\n\n\n\n\n<ol>\n\n\n<li><strong><a href=\"\/faculty\/gregory_taylor\/index.php?Page=5694\">Transcriptional regulation of abiotic stress responsive genes<\/a> (Ph.D.)<\/strong><\/li>\n\n\n\n\n<p>The student will undertake identification of <em>cis<\/em>-regulatory elements (<span style=\"font-style: italic\">c<\/span>REs) from the promoters of genes co-expressed under two commonly co-occurring abiotic stresses, aluminum and phosphate stress. Using one-hybrid systems, the proteins\/transcription factors which bind these sequences will be identified. The interaction will then be validated and further characterized through <span style=\"font-style: italic\">in vitro<\/span>\/<span style=\"font-style: italic\">in vivo<\/span> analyses. In addition, the student will identify additional <span style=\"font-style: italic\">c<\/span>RE containing genes using <span style=\"font-style: italic\">in silco<\/span> genome mining and validate their stress responsive expression, expanding the \u201cregulon\u201d of co-expressed genes beyond the initial set. Applicants should have background in plant physiology, molecular biology, and bioinformatics.<\/p>\n\n\n\n\n<li><strong><a title=\"\" href=\"\/faculty\/gregory_taylor\/?Page=5185\">Genetic regulation of cadmium accumulation in wheat<\/a> (M.Sc.)<\/strong><\/li>\n\n\n\n\n<p>The student will undertake functional analysis of genes that are potentially involved in regulating cadmium accumulation in durum wheat grain. Two complementary approaches will be use: yeast as a model system to examine how products encoded by candidate genes function at the cellular level; and virus-induced gene silencing (VIGS) to characterize the function of these candidates <span style=\"font-style: italic\">in planta<\/span>. This project is part of a larger collaborative effort that integrates transcriptomics and genetic mapping to clone and characterize genes responsible for low grain cadmium concentration in durum wheat. Applicants should have background in plant physiology and molecular biology.<\/p>\n\n<\/ol>\n\n\n\n\n<p>Interested students will need to meet the Department of Biological Sciences <a href=\"\/programs\/graduate\/prospective\/?Page=4511\">entry standards<\/a> and qualify for funding support (e.g. Departmental Teaching Assistantship, NSERC studentship). Additional information for prospective students is available on the <a href=\"\/programs\/graduate\/prospective\/\">Biological Sciences website<\/a>. Contact Dr. Taylor (<a href=\"mailto:gregory.taylor@ualberta.ca\">gregory.taylor@ualberta.ca<\/a>) for more information.<\/p>\n\n--><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Graduate Research Opportunity I am looking for a M.Sc. or Ph.D. student to undertake functional characterization of P1B-type heavy-metal ATPase transporters from Brachypodium distachyon or wheat. Details to be announced Master&#8217;s or Ph.D. student projects will address some of these questions and would be tailored to the student&#8217;s interests and background. A B.Sc. in plant [&hellip;]<\/p>\n","protected":false},"author":60,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-150","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/150","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/users\/60"}],"replies":[{"embeddable":true,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/comments?post=150"}],"version-history":[{"count":1,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/150\/revisions"}],"predecessor-version":[{"id":151,"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/pages\/150\/revisions\/151"}],"wp:attachment":[{"href":"https:\/\/grad.biology.ualberta.ca\/taylor\/wp-json\/wp\/v2\/media?parent=150"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}